Yielding Accuracy of Morning and Spot Sputum Samples for Diagnosis Mycobacterium Tuberculosis Using Gene Xpert MTB/RIF Assay at EPHI, Addis Ababa, Ethiopia

Background: Tuberculosis is a global health problem and leading cause of mortality and morbidity in the world. Correct diagnosis of tuberculosis is the main challenge for control of tuberculosis. World health organization has prioritized improving diagnostic guidelines and tests for TB diagnosis. Priority has been given for development of new Tuberculosis diagnostic methods. But diagnosis of Tuberculosis highly affected by quality specimen collected which has been given less attention. The aim of this is to assess Yielding accuracy of morning and spot sputum samples for diagnosis Mycobacterium tuberculosis using Gene Xpert Mycobacterium Tuberculosis (MTB)/Rifampicin Resistance (RIF).

Methodology: Cross sectional study was conducted and convenient sampling technique used to select participants. Checklists used to collect the data from April 1 to May 30, 2018 G.C. A total of two hundred twenty two (222) sputum samples from 111 suspected tuberculosis patients were analyzed using Gene Xpert MTB/RIF Assay. SPSS version 20 was used for statistical analysis. Results: From 111 TB suspected patients, 55.9% were male and the rest of 44.1% was female. In terms of sample quality, 81.1% morning sputum samples and 79.3% spot sputum samples were had good sample quality. The outcomes of the research show an increase in odds value in sputum quality and sputum consistency for TB diagnosis. But, the relation is not significant association
with TB positivity.

Conclusion: Based on the results of this study, the diagnosis of TB using spot sputum samples as well as using morning sputum samples is almost similar for detection of MTB by Gene Xpert MTB/RIF Assay. Physical Quality of sputum sample or consistency is not association with TB positivity using Gene Xpert MTB/RIF Assay.

Results: From 111 TB suspected patients, 55.9% were male and the rest of 44.1% was female. In terms of sample quality, 81.1% morning sputum samples and 79.3% spot sputum samples were had good sample quality. The outcomes of the research shows an increase in odds value in sputum quality and sputum consistency for TB diagnosis. But, the relation is not significant association with TB positivity.

Conclusion: Based on the results of this study, the diagnosis of TB using spot sputum samples as well as using morning sputum samples is almost similar for the detection of MTB by Gene Xpert MTB/RIF Assay. Physical Quality of sputum sample or consistency is not associated with TB positivity using Gene Xpert MTB/RIF Assay.