Search by keyword or author

Oligoasthenozoospermic and Normozoospermic Indian Males Yielded Similar Fertilization Capability by Intracytoplasmic Sperm Injection

Published: April 12, 2023

Authors

Ram Dayal

Keywords
Capacitati, Intra-cytoplasmic sperm injection, sperm concentration, Sperm motility, Normozoospermia, Igoasthenozo-ospermia

Abstract

Background: The present study evaluated the fertilization outcomes by intra-cytoplasmic sperm injection (ICSI) technique with oligoastheno-zoospermic (OAZ) compared to normozoospermic (NZ) semen.

Purpose: To find the fertilizing capability of human sperm in oligo-asthenozoospermic and normozoospermic semen samples.

Methods: Fresh semen ejaculates belonging to NZ and OAZ groups (n=50 in each group) patients attending the infertility clinics were evalu-ated for semen volume, sperm motility, and sperm concentration. Ferti-lization rates with ICSI along with post-capacitation motility and con-centration were studied in both groups.

Results: The motility and concentration in fresh semen samples patients of the NZ group (32.44±1.50%, 71.96±6.45 M/ml, respectively) were significantly (P<0.05) higher compared to the OAZ group (26.76±1.38, 10.31±0.48 M/ml, respectively).However, the results of our study re-vealed similar fertilization rates in-vitro by ICSI in the NZ (98%) and OAZ (98%) groups.

Conclusion: The in-vitro processing by the ICSI technique is an effective approach to achieving good fertilization rates in sub-fertile males.

References

  • Adiga, S.K., Jayaraman, V., Kalthur, G., Upadhya, D., Kumar, P., (2008), Declining semen quality among south Indian subfertile men: A retrospective study. J. Hum. Reprod. Sci. 1, 15–18.
  • Agarwal, A. Singh, A., Hamada, A., Kesari, K., (2011), Review Article: Cell Phones and Male Infertility: A Review of Recent Innovations in Technology and Consequences, Center for Reproductive Medicine, Cleveland Clinic, Cleveland, Ohio, United States, International Braz J Urol, Vol 37 (4): 432-454, July – August.
  • Agarwal, A., Gupta, S., & Sharma, R. (Eds.), (2016), Andrological Evaluation of Male Infertility.doi:10.1007/978-3-319-26797-5.
  • Andolz, P., Bielsa, M. A., Vila, J., (1999), Evolution of semen quality in North-eastern Spain: a study in 22,759 infertile men over a 36 year period. Hum Reprod;14:731–35.
  • Blay, R. M., Pinamang, A. D., Sagoe, A.E., Owusu, ED, A., Koney, N.K, Arko-Boham, B., (2020), Influence of Lifestyle and Environmental Factors on Semen Quality in Ghanaian Men.International Journal of Reproductive Medicine, Article ID 6908458.7 pages, https://doi.org/10.1155/2020/6908458.
  • Borges, E. Jr., Rossi, L. M., Locambo de Freitas, C. V., Guilherme, P., Carvalho S Bonetti, T.,  Iaconelli, A.,  Firmbach Pasqualotto, F., (2007), Fertilization and pregnancy outcome after intracytoplasmic injection with fresh or cryopreserved ejaculated spermatozoa. Fertility and Sterility, 87(2) 316–320.
  • Borges, E. Jr., Setti, A.S., Vingris, L., Figueira Rde, C., Braga, D.P., and Iaconelli, A. Jr., (2013), Intracytoplasmic morphologically selected sperm injection outcomes: the role of sperm preparation techniques. J Assist Reprod Genet 30:849-54.
  • Camila N, Ivan H. Y., Fabia L. V., Waldemar P. C., Emerson B. C., Caio P. B., (2018), Seminal profile of 23,504 patients over the years: 7 years of experience. JBRA Assisted Reproduction 22(4):286-288. doi: 10.5935/1518-0557.20180055.
  • Dain, L., Auslander, R., Dirnfeld, M., (2011), The effect of paternal age on assisted reproduction outcome. Fertil Steril; 95:1–8.
  • David Mortimer, (2000), Sperm Preparation Methods, From the Genesis Fertility Centre, Vancouver, BC, Canada, Journal of Andrology, Vol. 21, No. 3, May/June.
  • Findekleet, S., Radosa, J.C., Radosa, M.P., Mohamad Eid Hammadeh (2020), Correlation between total sperm count and sperm motility and pregnancy rate in couples undergoing intrauterine insemination. Sci Rep 10, 7555, https://doi.org/10.1038/s41598-020-64578-0.
  • Geoffroy-Siraudin, C., DieudonnĂ© Loundou, A., Romain, F., Achard, V., Courbière, B., HĂ©lène Perrard, M., Durand, P., Roberte Guichaoua, M., (2012), Decline of semen quality among 10 932 males consulting for couple infertility over a 20-year period in Marseille, France. Asian J Androl. 14:584–90. doi:10.1038/aja.2011.173.
  • Girsh, E., Katz, N., Genkin, L., Girtler, O., Bocker, J., Bezdin, S., Barr, I., (2008), Male age influences oocyte-donor program results. J Assist Reprod Genet; 25:137–43.
  • Jequier AM, (2004), Clinical andrology-still a major problem in the treatment of infertility. Hum Reprod 19(6): 1245-1249.
  • Jhuang, H.Y., Chung, H.C., Wang, D. I., Peng, K.C., Meng, En., Chien, C. W., and Chang, Y. P., (2021), Association of Obstructive Sleep Apnea With the Risk of Male Infertility in Taiwan, Original Investigation, Pulmonary Medicine. Jan; 4(1): e2031846; doi: 10.1001/jamanetworkopen.2020.31846.
  • Kumar N, Singh AK, (2015), Trends of male factor infertility, an important cause of infertility: A review of literature. J Hum Reprod Sci.8(4):191-96.
  • Macklon NS, Stouffer RL, Giudice LC, FauserBC, (2006), The science behind 25 years of ovarian stimulation for in vitro fertilization, Endocr Rev; 27:170.
  • Mahadevan & Trounson, (2021), Removal of the cumulus oophours from the human oocyte for in vitro fertilization. Fertil Steril, 1985; 43-263-267. http://dx.doi.org/10.1016/Soo15-0282(16)48383-4
  • Makler A., (1980), The improved ten-micrometer chamber for rapid sperm count and motility evaluation.FertilSteril; 33: 337-8.
  • Malvezzi, H., Sharma, R., Agarwal, A., Abuzenadah, A. M., & Abu-Elmagd, M., (2014), Sperm quality after density gradient centrifugation with three commercially available media: a controlled trial. Reproductive biology and endocrinology 12(121). https://doi.org/10.1186/1477-7827-12-121.
  • Mamdooh A., Al-Nasrawi, Loqman J., Tawfiq, (2020), Bacterial Infection and Seminal Fluid Parameters in Iraqi SubFertile Men. Indian Journal of Forensic Medicine & Toxicology, 14(3).
  • Manna, C., Barbagallo, F., Manzo, R., Rahman, A., Francomano, D., Calogero, AE., (2020), Sperm Parameters before and after Swim-Up of a Second Ejaculate after a Short Period of Abstinence. Journal of Clinical Medicine. 9(4):1029. https://doi.org/10.3390/jcm9041029.
  • Marimuthu, P., Kapilashrami, M.C., Misro, M, M., Singh, G., (2003), Evaluation of trend in semen analysis for 11 years in subjects attending a fertility clinic in India. Asian J Androl. 5:221–5.
  • Maya, W.C., Berdugo, J., Jaramillo, A.C., (2009), The effects of maleage on semen parameters: analysis of 1364 men attending an Andrologycenter. Aging Male 12, 100–103.
  • Mehta, A., Bolyakov, A., Schlegel, P.N., Paduch, D.A., (2015), Higher pregnancy rates using testicular sperm in men with severe oligospermia. FertilSteril104:1382–1387.
  • Micic S., Lalic N., Djordjevic D., Bojanic N., Bogavac-Stanojevic N., Busetto G.M., Virmani A., Agarwal A., (2019), Double-blind, randomised, placebo-controlled trial on the effect of L-carnitine and L-acetylcarnitine on sperm parameters in men with idiopathic oligoasthenozoospermia.Andrologia, 51, e13267.
  • MĂ­nguez-AlarcĂłn, L., Gaskins, J.A., (2018), Type of underwear worn and markers of testicular function among men attending a fertility center, Hum Reprod. 2018 Sep; 33(9): 1749–1756., doi: 10.1093/humrep/dey259.
  • Mishra, P., Negi M, P. S., Srivastava, M., Singh, K., and Singh, R., (2018), Decline in seminal quality in Indian men over the last 37 years. Reproductive Biology and Endocrinology 16:103.
  • Mukhopadhyay, D., Varghese, A.C., Pal, M., Banerjee, S.K., Bhattacharyya, A.K., Sharma, R.K., Agarwal, A., (2010), Semen quality and age-specific changes: A study between two decades on 3,729 male partners of couples with normal sperm count and attending an andrology laboratory for infertility-related problems in an Indian city. FertilSteril. 93:2247–54.
  • Nadia A.S. Aleisa, (2013), Semen characteristics of fertile and subfertile men in a fertility clinic and correlation with age. Journal of King Saud University-Science. 25: 63-71.
  • Nagy, Z.P., Verheyen, G., Tournaye, H., Van Steirteghem, A.C., (1998), Special applications of intracytoplasmic sperm injection: the influence of sperm count, motility, morphology, source and sperm antibody on the outcome of ICSI. Hum Reprod 13(Suppl 1): 143-154.
  • Nogueira, C., Yoshida, I. H., Vilarino, F. L., Carvalho, W. P., Cordts, E. B., & Barbosa, C. P., (2018), Seminal profile of 23,504 patients over the years: 7 years of experience. JBRA assisted reproduction, 22(4), 286–288. https://doi.org/10.5935/1518-0557.20180055.
  • Oliveira, J.B.A., Petersen, C.G., Mauri, A.L., Vagnini, L.D., Baruffi, R.L.R., Franco Jr., J.G., (2014), The effects of age on sperm quality: an evaluation of 1,500 semen samples, JBRA Assisted Reproduction; 18(2): 34-41, original article, doi: 10.5935/1518-0557.20140002.
  • Palermo, G.D., Neri, Q.V., Takeuchi, T., Rosenwaks, Z., (2009), ICSI: where we have been and where we are going, Seminars in Reproductive Medicine 27: 191–201.
  • Parazzini, E., Marchini, M., (1995), Tight underpants and trousers and risk of dyspermia, international journal of andrology, 18: 137-1 40.
  • Roberts, M., and Jarvi, K., (2019), Steps in the investigation and management of low semen volume in the infertile man.CUAJ3(6): 479-485.
  • Sanlier, N., and Yorusun,T.O., (2018), The Relationship between Infertility and Nutrition,Volume 5 | Issue 2, Journal of Nutrition and Health Sciences, bISSN: 2393-9060.
  • Shah, D., Pandiyan N., Pandiyan R., Kartheeswaran J., and Prakash B., (2020), Ejaculatory abstinence in semen analysis: does it make any sense?Therapeutic Advances in Reproductive Health. 14: 1-11.DOI: 10.1177/2633494120906882.
  • Shekha, S.A., Narkhede, P., Fatima, T., Bhople, K.S, (2019), Patterns of semen parameters in male partners of infertile couples attending rural medical college. Int. J. Clin. Diagn. Pathol 2 (2): 341-348.
  • Sidhu RS, Sharma RK, Kachoria S, Curtis C, Agarwal A, (1997), Reasons for rejecting potential donors from a sperm bank program. J Assist Reprod Genet 14 (6): 354-360.
  • Sukcharoen, N., Ngeamjirawat, J., Chanprasit, Y., (1994), A Aribarg A Comparison of Makler Counting Chamber and Improved NeubauerHemocytometer in Sperm Concentration Measurement, J Med Assoc Thai September.
  • Miyamoto, T. (2012), Review Article Male Infertility and Its Causes in Human, Hindawi Publishing Corporation Advances in Urology, Article ID 384520, 7 pages doi:10.1155/2012/384520.
  • Unisa S., (2010), Infertility and Treatment Seeking in India: Findings from District Level Household Survey. F, V & V IN OBGYN, MONOGRAPH, 59-65.
  • Tayawade, V & More, A., (2018), Effect of Occupational Environment on Male Infertility, International Journal of Current Research and Review DOI: http://dx.doi.org/10.31782/IJCRR.2020.SP99.
  • Xie, B., Zhu, W-jie, Huang Y-hua, (2013), Outcome of conventional IVF and ICSI on sibling oocytesin moderate oligoasthenozoospermia. Pak J Med Sci 29:1221–4. 10.12669/pjms.295.3716.
  • Zegers-Hochschild F, Adamson GD, De Mouzon J, Ishihara O, Mansour R, Nygren K (2009), ICMART and WHO international committee for monitoring assisted reproductive technology (ICMART) and the World Health Organization (WHO) revised glossary of ART terminology, 2009. Fertil Steril. 92(5):1520-4.
  • Zhu, Q-X., Meads, C., Lu, M-L., Wu, J-Q., Zhou, W-J., Gao, E-S., (2011), Turning point of age for semen quality: a population-based study in Chinese men. Fertil Steril; 96:572–6.

How to Cite

Ram Dayal. Oligoasthenozoospermic and Normozoospermic Indian Males Yielded Similar Fertilization Capability by Intracytoplasmic Sperm Injection. J. Multidiscip. Res. Healthcare. 2023, 09, 51-58
Oligoasthenozoospermic and Normozoospermic Indian Males Yielded Similar Fertilization Capability by Intracytoplasmic Sperm Injection

Current Issue

PeriodicityBiannually
Issue-1June
Issue-2December
ISSN Print2393-8536
ISSN Online2393-8544
RNI No.CHAENG/2014/57978

This work is licensed under a Creative Commons Attribution 4.0 International License.

Articles in Journal of Multidisciplinary Research in Healthcare by Chitkara University Publications are Open Access articles that are published with licensed under a Creative Commons Attribution- CC-BY 4.0 International License. Based on a work at https://jmrh.chitkara.edu.in/. This license permits one to use, remix, tweak and reproduction in any medium, even commercially provided one give credit for the original creation.

View Legal Code of the above-mentioned license, https://creativecommons.org/licenses/by/4.0/legalcode

View Licence Deed here https://creativecommons.org/licenses/by/4.0/

Creative Commons License

Journal of Multidisciplinary Research in Healthcare by Chitkara University Publications is licensed under a Creative Commons Attribution 4.0 International License.
Based on a work at https://jmrh.chitkara.edu.in/

Visibility, Memberships and Ethics